fa روش های تشخیص آزمایشگاهی Laboratory Methods مقاله پژوهشی Original Research Article <p style="text-align: justify;"><strong>Backround:</strong> The present study investigated the correlation between p53 gene codon 72 polymorphism and 6 other genetic single nucleotide polymorphisms (SNPs) in patients with cervical cancer infected by HPV.</p> <p style="text-align: justify;"><strong>Methods:</strong> 450 patients with cervical cancer (280 Squamous cell carcinoma and 170 Adenocarcinoma) were followed at hospitals in Iran from Dec. 2014 to Apr. 2015. Moreover, 100 age/sex-matched were used as the control group. HPV was detected by LINEAR ARRAY&reg; HPV Genotyping Test. Allelic frequency of 6 gene polymorphisms was detected by the amplification-refractory mutation system (ARMS).</p> <p style="text-align: justify;"><strong>Results:</strong> From 450 patients, 408 cases (90.66%) were positive for HPV. Four genotypes were observed as single infections (16, 18, 31, and 45). The most common genotypes were HPV-16 (73.52%), HPV-18 (23.28%), HPV-31 and 45 (3.17%), respectively. 306 samples were arginine-arginine homozygous (70.6% and 71.4% of adenocarcinoma and squamous cell carcinoma, respectively), 70 cases were arginine-proline heterozygous (17.6% of adenocarcinoma and 23.8% of squamous cell carcinoma), and 20 cases were as proline-proline homozygous (11.8% and 4.8% of adenocarcinoma and squamous cell carcinoma, respectively).</p> <p style="text-align: justify;"><strong>Conclusion: </strong>The prevalence of HPV was 84% and that was the estimation of the Global Burden among Iranian patients with cervical cancer (85% - 99%). There was no correlation between mutations in the p53 allele and the size/type of tumors, while we found a correlation between mutations in p53 alleles and age. Therefore, <em>XRCC1</em> G399A SNP and <em>TP53</em> G72C SNP were significantly correlated with the cervical cancer.</p> <p style="text-align: justify;"></p> <p><strong>Abbreviations:</strong></p> <p>HPV: Human papillomavirus; SNP: Single nucleotide polymorphism; CDKN1A:&nbsp;<em>c</em>ycling-dependent kinase inhibitor 1A; TP53: Tumor&nbsp;<a name="_GoBack"></a>protein p53 (also known as p53); P53c72: p53 gene codon 72; ATM: Ataxia-telangiectasia mutated; HDM2: Human double minutes 2 (also known as MDM2); LIG4: DNA ligase IV; XRCC1: X-ray repair cross-complementing 1; XRCC3: X-ray repair cross-complementing 3; TGFB1: Transforming Growth Factor Beta 1; HWE&rsquo;: Hardy-Weinberg equilibrium; FIGO stage: International Federation of Gynecology and Obstetrics tumor stage;&nbsp;</p> Cervical Cancer, Human Papillomavirus, Amplification-refractory mutation system, p53 gene, Single Nucleotide Polymorphisms 50 59 http://modernmedlab.com/browse.php?a_code=A-10-1-24&slc_lang=fa&sid=1 Nikta Sajjadi niki.sajjadi@yahoo.com 10031947532846001578 10031947532846001578 No CBMA, Centre of Molecular and Environmental Biology Department of Biology, University of Minho Campus of Gualtar, Braga, Portugal Reza Mirnejad rmirnejad@yahoo.com 10031947532846001579 10031947532846001579 No Molecular Biology Research Centre, Baqiyatallah University of Medical Sciences, Tehran, Iran Malike Sharif 10031947532846001580 10031947532846001580 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Vahhab Piranfar vahab.p@gmail.com 10031947532846001581 10031947532846001581 Yes Department of Biology, Farname Inc, Thornhill, ON, Canada Davood Zaeifi gene_d.zaeifi@hotmail.com 10031947532846001582 10031947532846001582 No Department of Biology, North-Tehran Branch , Islamic Azad University, Tehran, Iran