en بیوشیمی پزشکی Medical Biochemistry مقاله پژوهشی Original Research Article <p dir="ltr"><strong>Background and Objective:</strong> Recent studies have shown that not only the quantity, but also the quality of dietary fatty acids can affect lipid parameters. Elaidic acid is a major hydrogenated by-product of vegetable oil play a role in the development of cardiovascular disease, especially atherosclerosis, through changing lipid profile and increase in inflammation and inflammatory factors. On the other hand, the role of TNF-&alpha; is evident in inflammatory pathways and inflammation outcomes, which are involved in the initiation of atherosclerosis. Therefore, in the current study the effect of elaidic acid on TNF-&alpha; gene expression, was investigated. &nbsp;</p> <p dir="ltr"><strong>Method: </strong>RAW264.7 cells were treated with 100 and 200 &mu;M of elaidic acid for 12 h. Then, expression of TNF-&alpha; gene was assessed after RNA extraction and cDNA synthesis.&nbsp;</p> <p dir="ltr"><strong>Results: </strong>&nbsp;TNF-&alpha; gene expression in RAW264.7 macrophage cell line showed no significant difference for both concentrations compared to the control.&nbsp;</p> <p dir="ltr"><strong>Conclusion: </strong>These results indicated that elaidic acid affect the development and acceleration of atherosclerosis in other ways than through TNF-&alpha; nuclear receptor.&nbsp;</p> Cell culture, Elaidic acid, Atherosclerosis, Gene expression, Trans-fatty acid, TNF- α 23 28 http://modernmedlab.com/browse.php?a_code=A-10-1-19&slc_lang=en&sid=1 Hossein Yeganeh Montakhab 10031947532846001564 10031947532846001564 Yes Department of clinical biochemistry, Tehran University of Medical Science, Tehran, Iran Mohammad Dousti 10031947532846001565 10031947532846001565 No Department of clinical biochemistry, Tehran University of Medical Science, Tehran, Iran Hossein Baba Ahmadi Rezaie 10031947532846001566 10031947532846001566 No Department of clinical biochemistry, Tehran University of Medical Science, Tehran, Iran