Modern Medical Laboratory Journal

en Detection of Metallo-beta-lactamase Producers Among Clinical Isolates of Enterobacterales in a Tertiary Care Centre, Nepal میکروب شناسی بالینی Clinical Microbiology مقاله پژوهشی Original Research Article   Background and Objective: Increased reports of metallo-beta-lactamase (MBL)-producing isolates of Enterobacterales indicate that there are limited therapeutic options available for the treatment of infections caused by these organisms. To the best of our knowledge, there have been only a limited number of studies on MBL-producing Enterobacterales in Nepal. Therefore, this study was conducted to determine the current distribution of MBL-producing isolates in our setting. Methods: This was a cross-sectional study conducted over a period of six months (from August 2023 to January 2024) at the Department of Microbiology, TU Teaching Hospital, Nepal. A total of 243 clinical isolates of Enterobacterales from various clinical specimens were subjected to the Modified Carbapenem Inactivation Method (mCIM) and the EDTA-Modified Carbapenem Inactivation Method (eCIM) for the detection of MBL-producing isolates, according to the standard methodology outlined by the latest Clinical and Laboratory Standards Institute (CLSI) guidelines. Result: A total of 111 (45.70%) isolates were identified as potential carbapenemase producers, based on their resistance or intermediate susceptibility to carbapenems (Meropenem and/or Imipenem) and/or resistance to cephalosporin subclass III (Ceftazidime). Among the total isolates of Enterobacterales, 52 (21.40%) were confirmed as carbapenemase producers by the mCIM method, 45 (18.50%) were confirmed as MBL producers by the eCIM method, while the remaining 7 (2.90%) were identified as serine-carbapenemase producers. Multidrug resistance (MDR) was observed in 151 (62.10%) of the total isolates, and all MBL-producing isolates were also MDR. Conclusion: This study found a higher percentage of MBL-producing Enterobacterales isolates, along with a high prevalence of multidrug resistance. Regular surveillance and stringent infection control policies are essential to minimize the spread of resistant strains. carbapenemase,eCIM,Enterobacterales,mCIM,MDR,phenotypic detection of MBL. 12 21 http://modernmedlab.com/browse.php?a_code=A-10-922-1&slc_lang=en&sid=1 Bijaya Dhakal dhakalb538@gmail.com 10031947532846002242 10031947532846002242 Yes Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Kathmandu, Nepal Junu Richhinbung Rai dr.junurai@iom.edu.np 10031947532846002243 10031947532846002243 No Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Kathmandu, Nepal Shristi Raut rautshristi@gmail.com 10031947532846002244 10031947532846002244 No Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Kathmandu, Nepal Hari Prasad Kattel hariprasadkattel@gmail.com 10031947532846002245 10031947532846002245 No Department of Microbiology, Tribhuvan University Teaching Hospital, Institute of Medicine, Kathmandu, Nepal Sujata Thapa sujatathapa152@gmail.com 10031947532846002246 10031947532846002246 No Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Kathmandu, Nepal Marichman Bhul marichnirankari@gmail.com 10031947532846002247 10031947532846002247 No Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Kathmandu, Nepal Naresh Bahadur Khadka cls.naresh@gmail.com 10031947532846002248 10031947532846002248 No Department of Microbiology, Tribhuvan University Teaching Hospital, Institute of Medicine, Kathmandu, Nepal Sangita Sharma drsangita_sharma@iom.edu.np 10031947532846002249 10031947532846002249 No Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Kathmandu, Nepal