en روش های تشخیص آزمایشگاهی Laboratory Methods مقاله پژوهشی Original Research Article <strong>Objective:</strong> This study aims to estimate and compare the time-course change in blood glucose levels by blood cells in serum, and plasma with or without preservatives, which may reflect the rate of glucose utilization by blood cells.<br> <strong>Method:</strong> This laboratory-based cross-sectional study was carried out using a blood specimen of 28 participants among which 14 were diagnosed with diabetes and 14 were non-diabetic. Fasting blood specimen was collected in a plain tube, Ethylene Diamine Tetra Acetic Acid (EDTA) tube, and EDTA+ Sodium Fluoride (NaF) tube. The test was performed by hourly estimation of glucose for 24 hours. Time-course changes in glucose levels in serum and plasma with or without NaF preservative were statistically compared using ANOVA test.<br> <strong>Result:</strong> Serum and EDTA plasma glucose levels decreased gradually after the 3rd hour to 24th hour in comparison to EDTA+NaF plasma (p<0.05).&nbsp; The rate of glucose utilization by blood cells was significantly higher in clotted blood and anticoagulated blood (EDTA) specimens in comparison with anticoagulated blood (EDTA) containing preservative (NaF) ((p<0.05). In addition, decreased rate of glucose utilization was observed in hyperglycemic specimens compared to that of normoglycemic blood.<br> <strong>Conclusion:</strong> Higher rate of glucose utilization by blood cells observed in serum and EDTA plasma represents a pre-analytical error in a long-standing specimen. The use of preservative NaF with EDTA significantly prevents cellular glucose utilization and stabilize plasma glucose level.&nbsp; In contrast, this study also shows further insight into the reduced cellular metabolic rate of glucose utilization in diabetes mellitus. Blood glucose, Anticoagulants, Glucose utilization, Plasma, Storage Time 1 9 http://modernmedlab.com/browse.php?a_code=A-10-860-1&slc_lang=en&sid=1 Mahendra Prasad Bhatt mahendramlt@gmail.com 10031947532846001658 10031947532846001658 Yes Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences Sujina Shrestha sujina.stha123@gmail.com 10031947532846001659 10031947532846001659 No Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences Sushant Pokhrel sushant.pokhrel@ymail.com 10031947532846001660 10031947532846001660 No Department of Laboratory Medicine, Manmohan Memorial Institute of Health Sciences Prabodh Rijal prabodhrisal@kusms.edu.np 10031947532846001661 10031947532846001661 No Kathmandu University School of Medical Sciences Amar Nagila nagila2a@gmail.com 10031947532846001662 10031947532846001662 No Faculty of Health Sciences, Pokhara University, Kaski, Nepal