Modern Medical Laboratory Journal

Search published articles

Showing 2 results for Muris

Identification of Mycoplasma Muris Isolated from Vaginal Samples of NIH Mice

Mohammad Reza Zinatizadeh, Fatemeh Abedini, Mostafa Jafarpour, Zahra Masoumalinejad,
Volume 2, Issue 1 (1-2019)

Abstract

Mycoplasma muris (M.M) is a small pathogenic bacterium that lives in the female mouse genital tract. Mycoplasma muris may have harmful effects on the reproductive health of female. This research was performed to optimize the detection of M. muris in NIH mice in the Department of Animal Breeding, Razi Vaccine and Research Institute, Iran. In this cross-sectional study, 29 vaginal samples of NIH mice were selected through simple random sampling. For detection of the mycoplasma, the vaginal tissue removal of samples was done. First, samples were crushed using mortar and pestle with PBS 1ml, then were cultured in the PPLO broth and incubated at 37°C for 24h, they were passed through 0.45 μm pore-size filters and inoculated into specific PPLO broth and agar media for 3-4 weeks. In the next section, the PCR test was used with primers of 16S rRNA gene of M. muris. From 29 tested samples, 17.24% samples were positive for M. muris by PCR method, while 35.93% cultures showed positive. The phylogenetic analysis indicated a new strain of M. muris. The results of culture and PCR methods displayed the contamination in NIH mice. Therefore, Therefore, more researches are needed regarding the presence of mycoplasma for treatment and clinical signs.

A Review of Mycoplasma in Laboratory Mice

Zahra Masoumalinejad, Mohammad Reza Zinatizadeh, Najmeh Tahmasebiabdar,
Volume 2, Issue 2 (6-2019)

Abstract

Mycoplasma are small, cell-free bacteria enclosed by a membrane. These bacteria belong to the class of Mollicutes, the order of Mycoplasma tales, and the genus of Mycoplasma. There are more than 100 identified species of mycoplasma. The ratio of cytosine to guanine in its DNA is 23–40% and its genome size is 1350–600 kb. Mycoplasma require cholesterol to grow, and the temperature suitable for the growth of this bacteria is 37°C. Mycoplasma cause contamination and infections in humans and animals. Some mycoplasma species are seen only in animals. In general, mycoplasma are colonized at the surface of the mucus, and most species are non-invasive. Five main species of mycoplasma have been identified in laboratory mice, including: M.arthritidis, M.collis, M.muris, M.neurolyticum, and M.pulmonis. These species generally require protein-rich environments that contain 10–15% of the animal's serum, and their growth requires nicotinamide adenine nucleotide (NAD), which is commonly used to cultivate mycoplasma in mice. Laboratory research has found that mycoplasmas contamination has an adverse effect on animals. Therefore, it is important that health monitoring programs are implemented as a quality control for animals used in laboratory research.

Page 1 from 1