Modern Medical Laboratory Journal

Clustered Regularly Interspaced Short Palindromic Repeats, commonly known as CRISPR, has emerged as a groundbreaking gene-editing technology, revolutionizing the field of molecular biology. Originally discovered as part of the bacterial immune system, CRISPR has been adapted for precise manipulation of DNA sequences in various organisms, offering unprecedented opportunities in medicine, agriculture, and biotechnology. CRISPR was initially identified in bacteria as a defense mechanism against viral infections. The system consists of short, partially palindromic repeated DNA sequences interspersed with unique spacer sequences derived from past viral invasions. In conjunction with Cas proteins, CRISPR provides bacteria with the ability to recognize and eliminate specific viral DNA, creating a memory of past infections. The key to CRISPR's success lies in its molecular scissors – the Cas9 protein. When guided by a synthetic RNA molecule that matches a target DNA sequence, Cas9 precisely cleaves the DNA at the desired location. This targeted DNA break triggers cellular repair mechanisms, allowing for the introduction of specific genetic modifications.CRISPR has sparked a revolution in medical research and treatment. Its potential for precise gene editing opens avenues for developing therapies for genetic disorders, cancer, and infectious diseases. The ability to modify genes associated with hereditary conditions holds promise for personalized medicine, offering tailored treatments based on an individual's genetic makeup.
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